Journal: Chinese Medicine

Article Title: A clinical experience-based Chinese herbal formula improves ethanol-induced drunken behavior and hepatic steatohepatitis in mice models

doi: 10.1186/s13020-023-00753-5

Figure Lengend Snippet: The metabolic enzymes ADH1, ALDH2 and CYP2E1 are significantly up-regulated by alcohol, and further up-regulated by BGXJW administration. A – E The mRNA levels of ADH1, ALDH2, ALDH1, CYP2E1 in liver tissues were subjected to RT-qPCR analysis. E Levels of ADH1, ALDH2, CYP2E1 in liver lysates after indicated treatment were determined by western blot, F Liver tissue sections were subjected to immunohistochemistry analysis of CYP2E1. All data are expressed as the mean ± SEM (n = 3). nsP > 0.05, *P < 0.05 vs ctrl group; nsP > 0.05 vs EtOH group. Crtl contrl, EtOH ethanol model, BGXJW Bao-Gan-Xing-Jiu-Wan

Article Snippet: The antibodies used against CYP2E1, SCD1, FASN, and PPAR-α were purchased from Abcam (Massachusetts, USA); antibodies against ADH1 and ALDH2 were purchased from Boster (California, USA) and Cell Signaling Technology (Boston, USA) separately.

Techniques: Quantitative RT-PCR, Western Blot, Immunohistochemistry

Journal: Nutrients

Article Title: Functionally Relevant Differences in Plasma Fatty Acid Composition and Expression of Cytotoxic and Inhibitory NK Cell Receptors between Healthy Young and Healthy Elder Adults

doi: 10.3390/nu12123641

Figure Lengend Snippet: Expression of Killing Inhibitory Receptors (KIR) and activating receptors in NK cells derived from young adults and elders. The results represent the percentage mean and SD and Mean Channel Fluorescence Intensity (MFI) in linear units for each group ( n = 30). The statistical analysis was accomplished using the paired Student’s t -test in both parameters analysed.

Article Snippet: The antibodies anti-CD158e1/e2 KIR3DL1 (clone REA168), anti-KIR2DS4/CD158f (clone JJC11.6) were purchased from Miltenyi Biotech.

Techniques: Expressing, Derivative Assay, Fluorescence

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: CD99 , REA1174 , 50 , 130-121-078 , PE , Miltenyi Biotec.

Techniques: Imaging